LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1598..1694) /label=attL2 /note="attL2 (c)" misc_signal complement(1709..1728) /note="T7 Promoter/priming site (c)" primer_bind 1736..1752 /note="M13 Reverse priming site" gene 1865..2674 /note="Kanamycin resistance gene" rep_origin 2795..3468 /note="pUC origin" PCR_frame 669..1598 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1618) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1615) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1606..3471,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1576..1596 /note="TEV site" source 673..1575 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:54249 IMAGE:5750385" /tissue_type="Lung, Spleen, fetal, pooled" /clone_lib="NIH_MGC_122" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..1575 /gene="DPAGT1" /gene_synonym=ALG7 /gene_synonym=CDG-Ij /gene_synonym=D11S366 /gene_synonym=DGPT /gene_synonym=G1PT /gene_synonym=GPT /gene_synonym=UAGT /gene_synonym=UGAT /db_xref="GeneID:1798" /db_xref="HGNC:2995" /db_xref="MIM:191350" CDS 673..1575 /dnas_title="DPAGT1 protein" /gene="DPAGT1" /gene_synonym=ALG7 /gene_synonym=CDG-Ij /gene_synonym=D11S366 /gene_synonym=DGPT /gene_synonym=G1PT /gene_synonym=GPT /gene_synonym=UAGT /gene_synonym=UGAT /codon_start=1 /product="DPAGT1 protein" /protein_id="AAH47771.1" /db_xref="GI:28838623" /db_xref="GeneID:1798" /db_xref="HGNC:2995" /db_xref="MIM:191350" /translation="MIFLGFADDVLNLRWRHKLLLPTAASLPLLMVYFTNFGNTTIVV PKPFRPILGLHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFN LVELEGDCRDDHVFSLYFMIPFFFTTLGLLYHNWYPSRVFVGDTFCYFAGMTFAVVGI LGHFSKTMLLFFMPQVFNFLYSLPQLLHIIPCPRHRIPRLNIKTGKLEMSYSKFKTKS LSFLGTFILKVAESLQLVTVHQSETEDGEFTECNNMTLINLLLKVLGPIHERNLTLLL LLLQILGSAVTFSIRYQLVRLFYDV" misc_feature 673..1575 /note="DPAGT1 coding region" misc_difference 1528..1528 /gene="DPAGT1" /gene_synonym=ALG7 /gene_synonym=CDG-Ij /gene_synonym=D11S366 /gene_synonym=DGPT /gene_synonym=G1PT /gene_synonym=GPT /gene_synonym=UAGT /gene_synonym=UGAT /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgatctt cctgggcttt gcggatgatg tactgaatct gcgctggcgc 721 cataagctgc tgctacctac agctgcctca ctacctctcc tcatggtcta tttcaccaac 781 tttggcaaca cgaccattgt ggtgcccaag cccttccgcc cgatacttgg cctgcatctg 841 gacttgggaa tcctgtacta tgtctacatg gggctgctgg cagtgttctg taccaatgcc 901 atcaatatcc tagcaggaat taacggccta gaggctggcc agtcactagt catttctgct 961 tccatcattg tcttcaacct ggtagagttg gaaggtgatt gtcgggatga tcatgtcttt 1021 tccctctact tcatgatacc cttttttttc accactttgg gattgctcta ccacaactgg 1081 tacccatcac gggtgtttgt gggagatacc ttctgttact ttgctggcat gacctttgcc 1141 gtggtgggca tcttgggaca cttcagcaag accatgctac tattcttcat gccccaggtg 1201 ttcaacttcc tctactcact gcctcagctc ctgcatatca tcccctgccc tcgccaccgc 1261 atacccagac tcaatatcaa gacaggcaaa ctggagatga gctattccaa gttcaagacc 1321 aagagcctct ctttcttggg cacctttatt ttaaaggtgg cagagagcct ccagctggtg 1381 acagtacacc agagtgagac tgaagatggt gaattcactg aatgtaacaa catgaccctc 1441 atcaacttgc tacttaaagt ccttgggccc atacatgaga gaaacctcac attgctcctg 1501 ctgctgctgc agatcctggg cagtgccgtc accttctcca ttcgatatca gctcgttcga 1561 ctcttctatg atgtcgaaaa cttgtacttt caaggctCCA GCTTTCTTGT ACAAAGTTGG 1621 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 1681 TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT 1741 AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA 1801 TAAAATAATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG 1861 TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC 1921 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA 1981 TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT 2041 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT 2101 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT 2161 CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT 2221 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT 2281 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT 2341 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA 2401 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT 2461 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG 2521 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC 2581 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT 2641 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA 2701 CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA 2761 CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 2821 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 2881 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT 2941 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 3001 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 3061 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 3121 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 3181 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG 3241 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 3301 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 3361 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 3421 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T //